|
KMID : 0043320010240040355
|
|
Archives of Pharmacal Research
2001 Volume.24 No. 4 p.355 ~ p.359
|
|
|
Determination of Recombinant Human Epidermal Growth Factor (rhEGF) in a Pharmaceutical Formulation by High Perfor-manse Liquid Chromatography with Electrochemical Detection
|
|
Lee KW
Hwang KH/Kim CS/Chung YB/Park JS/Lee YM/Moon DC
|
|
|
Abstract
|
|
|
|
A novel HPLC method with electrochemical detection has been developed for the deter- mination of recombinant human epidermal growth factor (rhEGF) in pharmaceutical products. rhEGF was separated from other components in formulation on a reversed-phase Cl8 column with 24% acetonitrile in 0.1M phosphate buffer (Ph 4.75). The optimum electrochemical oxidation of EGF was obtained at 0.85 V vs. Ag/AgCl in a glassy carbon working electrode due to electroactive tyrosine, tryptophan, methionine, and arginine residues. The quantitation range was from 1.0 to 200 ng of rhEGF with the linear correlation coefficient greater than 0.999. The method was successfully applied for the quantitation of rhEGF in a pharmaceutical preparation.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|